Download PDF by Elmer H. Marth, James Steele: Applied Dairy Microbiology, Second Edition

By Elmer H. Marth, James Steele

ISBN-10: 082470536X

ISBN-13: 9780824705367

Reference and textual content supplying complete insurance of the most recent advancements and medical advances in dairy microbiology, with an emphasis on illness prevention, probiotics, fermented dairy items and regulatory keep watch over criteria for dairy meals.

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Additional info for Applied Dairy Microbiology, Second Edition

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Data based on Laver et al. 7 V2 V4 V5 V6 V7 V8 VlO VII V9 V12 V3 a The data are expressed as 100 cpm bound virus X/cpm bound PR/8. Another ten monoclonal antibodies could not distinguish between PR/8/34 and these 12 PEG-l variants, while a further three are not included since they displayed reactivity patterns represented by the hybridomas shown the peptide map analysis. The fmal antigenically distinct variant, V3, was found to have an amino acid substitution which resulted in the appearance of an additional soluble tryptic peptide presumably derived from a previously unmapped insoluble region.

1980a); Mem/102172 (Wardand Dopheide 1980a; Dopheide and Ward 1980a; Sleigh et al. 1980); Vic/317S (Min-Jou et al. 1980); Text V77 (Moss, unpublished; Laver et al. 1980a); Bang/1179 (Both and Sleigh 1981). Peptide data for Vic/ll7S showed Asp notAsn at residue 53 in HAl (Laveretal. 1980a). The amino acid sequence of the putative progenitor of the Hong Kong sub-type AlducklUkraine/1/63 (Hav1) is also shown (Ward and Dopheide 1981b, c; Ward et al. /77 8ang/79 Duck/Ukr /1/63 Aichl/2/68 (X-31)a NT/60/68 Alchl/2/68 (X-3l) b Hem/l/7!

These studies established that as expected many of the antibody speciftcities reflected in the reactivity patterns of the monoclonal antibodies represent overlapping epitopes on the hemagglutinin molecule and that the number of discrete antigenic determinants may be quite small. They also indicate that monoclonal antibodies represent a very powerful tool with which antigenic variants can be selected which should have amino acid sequence changes restricted to that region of the molecule that affects the determinant recognized by the particular antibody used in its selection.

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Applied Dairy Microbiology, Second Edition by Elmer H. Marth, James Steele


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