Download PDF by Mark D. Adams, Chris Fields, J. Craig Venter: Automated DNA Sequencing and Analysis

By Mark D. Adams, Chris Fields, J. Craig Venter

ISBN-10: 0080926398

ISBN-13: 9780080926391

ISBN-10: 0127170103

ISBN-13: 9780127170107

A well timed publication for DNA researchers, automatic DNA Sequencing and research reports and assesses the state-of-the-art of automatic DNA series analysis-from the development of clone libraries to the developmentof laboratory and neighborhood databases. It offers the methodologies and techniques of computerized DNA series research in a manner that enables them to be in comparison and contrasted. by way of taking a extensive view of the Read more...

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Additional resources for Automated DNA Sequencing and Analysis

Sample text

G rowth und er strong shaking for 16-20 h at 37°C 4. Killing the host cells by 2 h incubatio n at 50-60°C 5. Settl ing cells by keeping the plates for 2 days at room tem per ature 6. Evaporation of 70-80 % of the liquid ( I day at room temp er atur e) to increa se phage concentration depending whether 96-, 384-, or 864-well plates are used . SBH does not require more than 5 ~1 of CR per clone . Such PCR reactions can be prepared in 20 ~ l wells of 864-well plate s for a price of less tha n 10 cents each.

0 : :: ; ; .. : ; 0 ; 0 0 O' ; ; : ·· , ~ ::; ; : :: ; : · : ;0 :: · : · ,· · · ··•. , ··· : - f1, ;: : , : · : . :: : '. ' :: .. : .. : " 33 . ' 0 .. : : : .. 3 Hybridization accuracy of 7-mer probes with dolblotted cDNAs. A filter , generated by spotting peR products from rando m cDNA clones, is hybridized successively with the indicated pair of complementary probes. The filter contain s pairs of dots in the strips of six rows (rows 1-4, 2-5 , and 3-{j) produced by spotting twice samples from 18 96-well plates.

1992). Sequencing by hybridization (SBH) is one such example. Its development would probably never be funded in the 'old' biology . SBH is based on the fundamental chemistry of life. Because of the chemical specificity of base pairing, synthetic oligonucl eotides of known formulae are able to ' read' DNA sequences when hybridiz ed under full-match-sp ecific conditions . , 1988; Southern, 1988; Drmanac et al. , 1989; Macevicz, 1989). Nevertheless, the approach has experienced a few years of ignorance and skepticism.

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Automated DNA Sequencing and Analysis by Mark D. Adams, Chris Fields, J. Craig Venter


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