By Gérald Thouand, Robert Marks
This e-book assessment sequence offers present developments in glossy biotechnology. the purpose is to hide all elements of this interdisciplinary know-how the place wisdom, tools and services are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computing device technological know-how. Volumes are equipped topically and supply a complete dialogue of advancements within the respective box during the last 3-5 years. The sequence additionally discusses new discoveries and functions. detailed volumes are devoted to chosen themes which specialise in new biotechnological items and new techniques for his or her synthesis and purification. more often than not, detailed volumes are edited via famous visitor editors. The sequence editor and writer will even if constantly be happy to obtain feedback and supplementary details. Manuscripts are authorized in English.
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Extra resources for Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2
Philips NV (2011) Philips—design portfolio—design probes—bio-light. design. page. Accessed 7 August 2013 30 B. Reeve et al. 40. Pinheiro VB (2012) Synthetic genetic polymers capable of heredity and evolution. Science 336(6079):341–344 41. Pokhilko A et al (2012) The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops. Mol Syst Biol 8:574 42. Presidential commission for the study of bioethical issues (2010) New directions: the ethics of synthetic biology and emerging technologies 43.
2 mL stable light ATP reagent with 50 U/mL hexokinase was added. e. I–glucose and I+glucose). The unknown ATP concentration was calculated from the known glucose concentration (20 lmol/L) by multiplying it with IÀglucose =ðIÀglucose ÀIþglucose ). We used rather high concentrations of ATP and glucose to reach the endpoint as quickly as possible. The ATP concentration would actually have been outside the linear range of the luciferase reaction, if the competitive inhibitor AMP had not been included.
Nat Rev Genet 11(5):367–379 27. Koksharov MI, Ugarova NN (2011) Thermostabilization of firefly luciferase by in vivo directed evolution. Protein Eng Des Sel 24(11):835–844 28. Koncz C et al (1987) Expression and assembly of functional bacterial luciferase in plants. Proc Natl Acad Sci USA 84(1):131–135 29. Krichevsky A et al (2010) Autoluminescent plants (Uversky VN ed). e15461 30. Leclerc GM et al (2000) Development of a destabilized firefly luciferase enzyme for measurement of gene expression.
Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2 by Gérald Thouand, Robert Marks