By Lester Packer (Eds.)
The seriously acclaimed laboratory typical, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. for the reason that 1955, each one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this day - really an important booklet for researchers in all fields of existence sciences
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Additional info for Biomembranes Part I: Visual Pigments and Purple Membranes II
Reconstitution and Reduction of HR To L-O7 vesicles in 4 M sodium chloride (10-50 mg/ml protein) [aH]. 5 nmol halorhodopsin per 10 nag of protein). 0, containing 2% cyanoborohydride is added. After addition of a saturating amount of diethyl ether the sample is incubated in the dark at room temperature for 3 hr. This treatment produces a covalent linkage between the retinal moiety and HO and the product can be identified on SDS-gel by fluorography,n Reconstitution of BR in Apomembranes and White Membranes The reconstitution reaction of BR in apomembranes has been analyzed in great detail.
Caplan, in "Molecular Aspects of Membrane Transport" (D. Oxender and C. F. ), p. 553. Alan R. , New York, 1978. 20 C. Pasternak and M. Shinitzky, in "Energetics and Structure of Halophilic Microorganisms" (S. R. Caplan and M. ), p. 309. Elsevier/North-Holland Biomedical Press, Amsterdam, 1978. 21 B. D. Ladbrooke, R. M. Williams, and D. Chapman, Biochim. Biophys. Acta 150, 333(1968). ~2 E. Racker and P. C. Hinkle, J. Membr. Biol. 17, 181(1974). 23 R. J. Cherry, U. MOiler, R. Henderson, and M. P.
If this solution is not colorless, the cholate salt should be pretreated with active charcoal. 8 Sodium cholate from Merck ("for biochemical purposes") does not require this treatment. Phospholipids. L-Dilauroylphosphatidylcholine (DLPC), L-dimyristoylphosphatidylcholine (DMPC), L-dipalmitoylphosphatidylcholine (DPPC) from Calbiochem. Egg phosphatidylcholine (EPC) either from Sigma (type VE) or from Lipid Products (London). Alternatively, EPC can be isolated from egg yolk. 5 ml chloroform-methanol = 2:1 (by volume).
Biomembranes Part I: Visual Pigments and Purple Membranes II by Lester Packer (Eds.)