By Sidney Fleischer, Becca Fleischer (Eds.)
The significantly acclaimed laboratory normal, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this present day - really an important booklet for researchers in all fields of lifestyles sciences
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S. Labiner, E. Rabon, A. P. Douglas, M. W. Walling, and E. M. Wright, J. Membr. Biol. 50, 543 (1979). 23 R. J. Pennington, Biochem. J. 80, 649 (1961). " Bio-Rad Laboratories, Richmond, California. 30 MEMBRANE SEPARATION  TABLE I TYPICAL MEMBRANEMARKERS Marker Principle location Na+,K÷-ATPase (K+-pNPPase) Basolateral membranes Suerase and maltase Brush border membranes Alkaline phosphatase y-Glutamyltransferase Galactosyltransferase Acid phosphatase Apical membranes Apical membranes Trans Golgi elements Various intracellular membranes N-Acetyl-fl-D-glucosaminidase NADPH-cytochrome-c reductase Lysosomes Endoplasmic reticulum Succinate dehydrogenase Protein Mitochondria Notes Ref.
Mircheff, C. C. Lu, and C. N. Conteas, Am. J. Physiol. 24S, G661 (1983). 3 A. K. Mircheff and C. C. Lu, Am. J. Physiol. 247, G651 (1985). 4 C. N. Conteas, A. A. McDonough,T. R. Kozlowski,C. B. Hensley,and A. K. Mircheff, Am. J. Physiol. 250, C430 (1986). 5 A. A. McDonough and A. K. Mircheff, Fed. , Fed. Am. Soc. Exp. Biol. 42, 1934 (1983). 6 A. K. Mircheff, D. J. Ahnen, A. Islam, N. A. Santiago, and G. M. Gray, J. Membr. Biol. 83, 95 (1985). 7 A. K. Mircheff, H. E. Ives, V. J. Yee, and D. G. Warnock, Am.
Mircheff, Fed. , Fed. Am. Soc. Exp. Biol. 42, 1934 (1983). 6 A. K. Mircheff, D. J. Ahnen, A. Islam, N. A. Santiago, and G. M. Gray, J. Membr. Biol. 83, 95 (1985). 7 A. K. Mircheff, H. E. Ives, V. J. Yee, and D. G. Warnock, Am. J. Physiol. 246, F853 (1984). METHODS IN ENZYMOLOGY,VOL. 172 Copyright © 1989by AcademicPress, Inc. All rights of reproductionin any form reserved. 5. Density gradient media differ from this only in their sorbitol concentrations. 5; it is diluted 5-fold during preparation of the isolation buffer and density gradient media.
Biomembranes Part S by Sidney Fleischer, Becca Fleischer (Eds.)