John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca's Biomembranes Part W: Cellular and Subcellular Transport: PDF

By John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca Fleischer

ISBN-10: 0121820939

ISBN-13: 9780121820930

The delivery volumes of the Biomembranes sequence have been initiated with Volumes a hundred twenty five and 126 of equipment in Enzymology, which coated shipping in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 persisted the topic with ATP-Driven Pumps and comparable delivery. mobile and Subcellular delivery: Eukaryotic (Nonepithelial) Cells used to be the subject of Volumes 173 and 174. The subject matter of this quantity, in addition to of quantity 192, is mobile and Subcellular shipping: Epithelial Cells

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196, 133 (1968). [ 1] SALIVARY SECRETION 9 humidified organ bath, which is maintained at 37 ° . , for 02 measurements), it is advisable to cannulate the facial vein both proximal and distal to the junction with the glandular artery. This avoids obstructing the venous outflow in case the rate of collection is not matched exactly by the rate of outflow. ). This is best done after the gland is peffused in vitro. The technique of in situ followed by in vitro perfusion ensures constant peffusion of the gland with blood or perfusate and it therefore avoids any period of anoxia.

Young, J. Membr. Biol. 84, 239 (1985). 4oA. J. Howorth, R. M. Case, and M. C. Steward, PfluegersArch. 408, 209 (1987). , Na + and C1-) can be studied noninvasively on intact glands by means of nuclear magnetic resonance (NMR). 4~-43Even though studies of intact salivary glands have wide applications, there are some limitations. Salivary glands comprise several morphologicaUy and functionally distinct epithelia-- secretory endpieces and a series of ducts? ,6 Thus, if one wishes to study, for example, ion transport processes and their regulation at the cellular level, it becomes desirable to separate the epithelia and to gain access to the basolateral and lumenal membranes and to the cellular compartment.

The effect of the PD-measuring electrode can be calculated exactly if the space occupied by the electrode remains constant during the measurement, as shown in the experimental set-up of Fig. 3. Equations (1) and (3) then have to be modified: R2m= (47~23APDoRdOd) [r2/(r 2 -- re2)] r ~ = (¢x~P,t/-/TEAPDo)+ r [ (4) (5) Both-Sides-Open Tubular Structure. When introducing current I intralumenally in the middle of a tubular segment (see Fig. 2), only 50% of the total amount of I spreads out to each end of the tubular structure.

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Biomembranes Part W: Cellular and Subcellular Transport: Epithelial Cells by John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca Fleischer

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